Regulation of gene expression in post-meiotic male germ cells: CREM-signalling pathways and male fertility.
Résumé
Spermatogenesis is a remarkably complex process in which diploid spermatogonial stem cells undergo a series of mitotic and meiotic cell divisions to give rise to haploid round spermatids. These haploid cells then go through a dramatic morphological remodelling involving extensive chromatin condensation, reduction in nuclear and cytoplasmic volume, formation of an acrosome system and tail, all of which contribute to the formation of a mature spermatozoon fully capable of fertilizing the oocyte and passing along its genetic information to the next generation. To accomplish such a complex program, an intricate and efficient mechanism is required to finely tune the levels of expression of specific genes necessary for this process. Accordingly, the regulation of gene expression in post-meiotic male germ cells is governed by specific mechanisms unique to these cells. The cyclic adenosine monophosphate (cAMP) response element modulator (CREM) is an essential component of this program, and its activity is regulated through interactions with a germ cell-specific, CREM phosphorylation-independent transcriptional co-activator, activator of CREM in testis (ACT). In turn, the ability of ACT to regulate CREM activity is controlled by a germ cell-specific kinesin, Kif17b, which regulates the subcellular distribution of ACT. Further, the mRNA from CREM target genes interacts with several germ cell-specific RNA-binding proteins, which function to transport and stabilize these mRNAs. This sophisticated and complex regulation of gene expression in post-meiotic germ cells is governed by unique mechanisms specific to these cells and is fundamental to male fertility.