Proteolytic cleavage of ALF into alpha- and beta-subunits that form homologous and heterologous complexes with somatic TFIIA and TRF2 in male germ cells.

Raffaella Catena (1) , Manuela Argentini (1) , Igor Martianov (1) , Catherine Parello (1) , Stefano Brancorsini (1) , Martti Parvinen (1) , Paolo Sassone-Corsi (1) , Irwin Davidson (1)

1 IGBMC - Institut de génétique et biologie moléculaire et cellulaire

Male germ cells specifically express paralogues of components of the general transcription apparatus including ALF a paralogue of TFIIAalpha/beta. We show that endogenous ALF is proteolytically cleaved to give alpha- and beta-subunits and we map the proteolytic cleavage site by mass spectrometry. Immunoprecipitations show that ALFalpha- and beta-subunits form a series of homologous and heterologous complexes with somatic TFIIA which is coexpressed in male germ cells. In addition, we show that ALF is coexpressed in late pachytene spermatocytes and in haploid round spermatids with transcription factor TRF2, and that these proteins form stable complexes in testis extracts. Our observations highlight how cleavage of ALF and coexpression with TFIIA and TRF2 increases the combinatorial possibilities for gene regulation at different developmental stages of spermatogenesis.

Domaines: Biologie moléculaire

Raffaella Catena, Manuela Argentini, Igor Martianov, Catherine Parello, Stefano Brancorsini, et al.. Proteolytic cleavage of ALF into alpha- and beta-subunits that form homologous and heterologous complexes with somatic TFIIA and TRF2 in male germ cells.. FEBS Letters, 2005, 579 (16), pp.3401-10. ⟨10.1016/j.febslet.2005.04.083⟩. ⟨hal-00187482⟩