Isolation, characterization and antiplasmodial activity of steroidal alkaloids from Funtumia elastica (Preuss) Stapf.

Guédé Noël Zirihi , Philippe Grellier (1) , Frédérick Guédé-Guina , Bernard Bodo (2) , Lengo Mambu (2)

1 MCAM - Molécules de Communication et Adaptation des Micro-Organismes
2 Laboratoire de chimie et biochimie des substances naturelles

Bioassay-guided fractionation of the EtOH extract of the stem bark of Funtumia elastica resulted in the isolation of four steroidal alkaloids, holarrhetine (1), conessine (2), holarrhesine (3) and isoconessimine (4). Their structures were determined on the basis of 1D- and 2D-NMR techniques and mass spectrometry. Compounds 1-4 exhibited in vitro antiplasmodial activity against the chloroquine-resistant strain FcB1 of Plasmodium falciparum with IC50 values ranging from 0.97 to 3.39 microM. They showed weak cytotoxicity against a rat cell line L-6 with IC50 values ranging from 5.13 to 36.55 microM.

Domaines: Biochimie, Biologie Moléculaire

Guédé Noël Zirihi, Philippe Grellier, Frédérick Guédé-Guina, Bernard Bodo, Lengo Mambu. Isolation, characterization and antiplasmodial activity of steroidal alkaloids from Funtumia elastica (Preuss) Stapf.. Bioorganic and Medicinal Chemistry Letters, 2005, 15 (10), pp.2637-40. ⟨10.1016/j.bmcl.2005.03.021⟩. ⟨hal-00171107⟩