The proinflammatory mediator Platelet Activating Factor is an effective substrate for human group X secreted phospholipase A2. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochimica et Biophysica Acta - Molecular Cell Research Année : 2006

The proinflammatory mediator Platelet Activating Factor is an effective substrate for human group X secreted phospholipase A2.

Résumé

Platelet Activating Factor (PAF) is a potent mediator of inflammation whose biological activity depends on the acetyl group esterified at the sn-2 position of the molecule. PAF-acetylhydrolase (PAF-AH), a secreted calcium-independent phospholipase A(2), is known to inactivate PAF by formation of lyso-PAF and acetate. However, PAF-AH deficient patients are not susceptible to the biological effects of inhaled PAF in airway inflammation, suggesting that other enzymes may regulate extracellular levels of PAF. We therefore examined the hydrolytic activity of the recently described human group X secreted phospholipase A(2) (hGX sPLA(2)) towards PAF. Among different sPLA(2)s, hGX sPLA(2) has the highest affinity towards phosphatidylcholine (PC), the major phospholipid of cellular membranes and plasma lipoproteins. Our results show that unlike group IIA, group V, and the pancreatic group IB sPLA(2), recombinant hGX sPLA(2) can efficiently hydrolyze PAF. The hydrolysis of PAF by hGX sPLA(2) rises abruptly when the concentration of PAF passes through its critical micelle concentration suggesting that the enzyme undergoes interfacial binding and activation to PAF. In conclusion, our study shows that hGX sPLA(2) may be a novel player in PAF regulation during inflammatory processes.

Dates et versions

hal-00169124 , version 1 (31-08-2007)

Identifiants

Citer

Sarah Gora, Gerard Lambeau, James G Bollinger, Michael Gelb, Ewa Ninio, et al.. The proinflammatory mediator Platelet Activating Factor is an effective substrate for human group X secreted phospholipase A2.. Biochimica et Biophysica Acta - Molecular Cell Research, 2006, 1761 (9), pp.1093-9. ⟨10.1016/j.bbalip.2006.08.004⟩. ⟨hal-00169124⟩
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