Poor recognition of HIV-1 Nef protein by CD8 T cells from HIV-1-infected children: impact of age.

Florence Buseyne , Daniel Scott-Algara (1) , Béatrice Corre (2, 3) , Françoise Porrot (4) , Elizabeth Monchatre , Nassima Bellal , Marianne Burgard , Christine Rouzioux , Stéphane Blanche , Yves Rivière (5)

1 Régulation des Infections Rétrovirales
2 Immunologie Moléculaire
3 Signalisation des Cytokines - Cytokine Signaling
4 Virus et Immunité
5 Immunopathologie Virale

Recognition of various HIV proteins by CD8 T cells from HIV-infected children was determined by two functional assays. First, using an Elispot assay, we show that 80% of patients recognized Gag, 77% recognized Pol, 61% recognized Env, 44% recognized Nef and 29% recognized Vif. Frequencies of Gag-, Pol-, and Env-specific IFN-gamma producing CD8 T cells were higher than frequencies of Nef and Vif-specific CD8 T cells. The poor recognition of Nef by ex vivo CD8 T cells was confirmed by CTL assays performed in HAART na? children: 25% of children had positive response against Nef versus 44, 63 and 62% for Env, Gag, and Pol, respectively. Memory Gag-specific CTL were positively correlated with age, whereas Nef-specific CTL were negatively correlated with age. The poor Nef-specific CD8 T cell response in HIV-infected children contrasts with dominance of Nef-specific responses in infected adults.

Domaines: Virologie

Florence Buseyne, Daniel Scott-Algara, Béatrice Corre, Françoise Porrot, Elizabeth Monchatre, et al.. Poor recognition of HIV-1 Nef protein by CD8 T cells from HIV-1-infected children: impact of age.. Virology, 2006, 354 (2), pp.271-9. ⟨10.1016/j.virol.2006.07.014⟩. ⟨hal-00167682⟩