The occurrence of CGRP like molecule in a siki ( Centroscymnus coelolepsis) hydrolysate of industrial origin

Oscar Martinez-Alvarez (1) , C. Delannoy , L. Guimas , Martine Fouchereau-Peron (1, 2)

1 Station de Biologie Marine de Concarneau
2 BOME - Biologie des organismes marins et écosystèmes

Fish protein hydrolysates (FPH) may have potential as bioactive components in functional foods as nutraceuticals. This study focused on the identification of calcitonin gene-related peptide (CGRP) molecules in FPH. CGRP is a neuropeptide belonging to the calcitonin/CGRP family, and is known as potent arterial and venous vasodilator in humans. Hydrolysates of industrial origin were prepared from siki (/Centroscymnus coelolepsis/) heads and analyzed for the presence of CGRP-like molecules using specific radioimmunoassays and radioreceptorassays. The biological activity of the CGRP-related molecules was assessed by their ability to stimulate the adenylate cyclase activity in rat liver membranes. They were finally purified using gel exclusion chromatography and HPLC. These molecules presented a molecular weight around 1500-2500 Da and were obtained with a purification factor of 79. The incorporation of FPH with CGRP-like molecules in functional foods could lead to develop new useful products for health and nutrition markets.

Mots clés: Functional foods; siki; CGRP; protein hydrolysate; bioactive peptides

Domaines: Biochimie [q-bio.BM]; Biotechnologies; Biotechnologie

Oscar Martinez-Alvarez, C. Delannoy, L. Guimas, Martine Fouchereau-Peron. The occurrence of CGRP like molecule in a siki ( Centroscymnus coelolepsis) hydrolysate of industrial origin. Journal of Agricultural and Food Chemistry, 2007, 55, pp.5469-5475. ⟨hal-00147242⟩