Protein detection based on microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)](2) solid contact and immobilized proteinases: Preliminary investigations
Abstract
Protein selective potentiometric microelectrodes with the PPy[3,3-Co(1,2-C2B9H11)(2) solid internal contact were fabricated by the immobilization of different proteinases such as trypsin, pronase E and carboxypeptidase B on the surface of platinum microelectrodes modified by a conducting polymer. The performance of these biosensors was investigated by using potentionnetric measurements. The feasibility of this biosensor for protein determination was demonstrated, with a detection limit of about 1-2 mu g/ml for prolamine in buffer solution.