Production of maltodextrin 1-Phosphate by Fibrobacter succinogenes S85

Régis Nouaille (1, 2) , Maria Matulova (1, 3) , A.M. Delort (1) , Evelyne Forano (2)

1 SEESIB - Synthèse et étude de systèmes à intêret biologique
2 MIC - Unité de Microbiologie
3 Institute of Chemistry

We show for the first time the occurrence of maltodextrin-1-Phosphate (MD-1P) (DP 2) in F. succinogenes S85, a rumen bacterium specialized in cellulolysis which is not able to use maltose and starch. MD-1P were found in intra and extracellular medium of resting cells incubated with glucose. We used 2D 1H NMR technique and TLC to identify their structure and quantify their production with time. It was also shown that these phosphorylated oligosaccharides originated both from exogenous glucose and endogenous glycogen

Mots clés: Rumen; Maltodextrin; Maltodextrin-phosphate; NMR; Fibrobacter succinogenes

Domaines: Autre

Régis Nouaille, Maria Matulova, A.M. Delort, Evelyne Forano. Production of maltodextrin 1-Phosphate by Fibrobacter succinogenes S85. FEBS Letters, 2004, 576, pp.226-230. ⟨10.1016/j.bebslet.2004.09.019⟩. ⟨hal-00136126⟩