Nitrile biotransformationby aspergillus niger

Radka Snajdrova (1) , Veronika Kristova-Mylerova (1) , Dominique Crestia (2) , Konstantina Nikolaou (1) , Marek Kuzma (1) , Marielle Lemaire (2) , Estelle Gallienne (2) , Jean Bolte (2) , Karel Bezouska (3) , Vladimir Kren (1) , Ludmila Martinkova (1)

1 Laboratory of Biotransformation
2 SEESIB - Synthèse et étude de systèmes à intêret biologique
3 Department of biochemistry

A nitrile-converting enzyme activity was induced in Aspergillus niger K10 by 3-cyanopyridine. The whole cell biocatalyst was active at pH 3–11 and hydrolyzed the cyano group into acid and/or amide functions in benzonitrile as well as in its meta- and para-substituted derivatives, cyanopyridines, 2-phenylacetonitrile and thiophen-2-acetonitrile. Amides constituted a significant part of the total biotransformation products of 2- and 4-cyanopyridine, 4-chlorobenzonitrile, 4-tolunitrile and 1,4-dicyanobenzene, while α-substituted acrylonitriles gave amides as the sole products

Mots clés: Aspergillus niger; Nitrile-converting enzymes; Nitrile hydratase; Nitrilase; Amidase

Domaines: Chimie organique

Radka Snajdrova, Veronika Kristova-Mylerova, Dominique Crestia, Konstantina Nikolaou, Marek Kuzma, et al.. Nitrile biotransformationby aspergillus niger. Journal of Molecular Catalysis B: Enzymatic, 2004, 29, pp.227-232. ⟨10.1016/j.molcatb.2003.12.012⟩. ⟨hal-00135932⟩