The in vitro osteoclastic degradation of nacre.

Denis Duplat (1) , A. Chabadel , M. Gallet , Sophie Berland (1) , Laurent Bédouet (1) , Marthe Rousseau (1) , S. Kamel , Christian Milet (1) , P. Jurdic , M. L. Brazier , Evelyne Lopez (1)

1 BOME - Biologie des organismes marins et écosystèmes

Osteoclast activity was studied on nacre, the mother of pearl (MOP) in order to assess the plasticity of bone resorbing cells and their capacity to adapt to a biomineralized material with a different organic and mineral composition from that of its natural substrate, bone. Pure MOP, a natural biomineralized CaCO(3) material, was obtained from Pinctada oyster shell. When implanted in the living system, nacre has proven to be a sustainable bone grafting material although a limited surface degradation process. Osteoclast stem cells and mature osteoclasts were cultured on MOP substrate and osteoclast precursor cells were shown to differentiate into osteoclasts capable of resorbing nacre substrate. However, analysis of the organization of the cytoskeleton showed that both a sealing zone and a podosome structure were observed on the nacre substrate. Moreover, MOP resorption efficiency was consistently found to be lower than that of bone and appeared to be a limited process.

Mots clés: Nacre; Bone graft; Osteoclast; Adhesion; Biodegradation

Domaines: Biochimie, Biologie Moléculaire

Denis Duplat, A. Chabadel, M. Gallet, Sophie Berland, Laurent Bédouet, et al.. The in vitro osteoclastic degradation of nacre.. Biomaterials, 2007, 28 (12), pp.2155-2162. ⟨10.1016/j.biomaterials.2007.01.015⟩. ⟨hal-00131756⟩