A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture.

Laurent Camborde (1) , Vincent Tournier (1) , Mildred Noizet (1) , Isabelle Jupin (1)

1 IJM (UMR_7592) - Institut Jacques Monod

Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfect Arabidopsis cells. Zeocin-resistant Arabidopsis calli were used to generate a suspension cell culture. Detection of viral proteins and RNAs after 18 months in culture demonstrated persistent replication of the replicon. The Arabidopsis cell culture yielded soluble, active replication complexes, providing a useful tool to study host factors involved in TYMV replication.

Mots clés: Turnip yellow mosaic virus; Selectable replicon; Replication complex; Arabidopsis suspension culture; Zeocin

Domaines: Microbiologie et Parasitologie; Virologie

Laurent Camborde, Vincent Tournier, Mildred Noizet, Isabelle Jupin. A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture.. FEBS Letters, 2007, 581 (2), pp.337-341. ⟨10.1016/j.febslet.2006.12.045⟩. ⟨hal-00125400⟩