Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors

Didier Casanova (1) , D. Giaume (2) , Tina Amirtha (1) , Thierry Gacoin (2) , Jean-Pierre Boilot (2) , Antigoni Alexandrou (1)

1 LOB - Laboratoire d'optique et biosciences
2 LPMC - Laboratoire de physique de la matière condensée

We have demonstrated fluorescence resonance energy transfer (FRET) between lanthanide-ion doped oxide nanoparticles acting as donors and organic acceptor molecules (Cy5). Due to the long nanoparticle lifetime and the large Stokes shift between nanoparticle absorption and emission, unambiguous and precise FRET measurements can be performed despite the presence of large free acceptor oncentrations. We determined FRET efficiencies as a function of Cy5 concentration which are in very good agreement with a multiple acceptor-multiple donor calculation. © (2006) COPYRIGHT SPIE

Mots clés: Absorption; Fluorescence resonance energy transfer; Lanthanides; Molecules; Nanoparticles

Didier Casanova, D. Giaume, Tina Amirtha, Thierry Gacoin, Jean-Pierre Boilot, et al.. Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors. Colloidal Quantum Dots for Biomedical Applications, Jan 2006, San José, United States. ⟨10.1117/12.646479⟩. ⟨hal-00102775⟩