Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii Λ-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis.

M. Guibet (1) , N. Kervarec , S. Génicot , Y. Chevolot , W. Helbert

1 SOL - Signaux oligosaccharidiques et lipidiques

lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora lambda-carrageenase. The main digestion products were fractionated and analysed by (1)H and (13)C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the lambda-carrageenase cleaves the beta-(1-->4) glycosidic bonds. (1)H and (13)C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii lambda-carrageenan.

Domaines: Médecine humaine et pathologie

M. Guibet, N. Kervarec, S. Génicot, Y. Chevolot, W. Helbert. Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii Λ-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis.. Carbohydrate Research, 2006, 341, pp.1859-1869. ⟨10.1016/j.carres.2006.04.018⟩. ⟨hal-00092454⟩