Mitochondrial localization of Arabidopsis thaliana Isu Fe–S scaffold proteins

S. Léon (1) , B. Touraine (2) , J.F. Briat (2) , S. Lobréaux (2)

1 Section of Molecular Biology, Division of Biological Sciences
2 BPMP - Biochimie et Physiologie Moléculaire des Plantes

Isu are sca.old proteins involved in iron–sulfur cluster biogenesis and playing a key role in yeast mitochondria and Escherichia coli. In this work, we have characterized the Arabidopsis thaliana Isu gene family. AtIsu1,2,3 genes encode polypeptides closely related to their bacterial and eukaryotic counterparts. AtIsu expression in a Saccharomyces cerevisiae Disu1 Dnfu1 hermosensitive mutant led to the growth restoration of this strain at 37 0C. Using Isu-GFP fusions expressed in leaf protoplasts and immunodetection in organelle extracts, we have shown that Arabidopsis Isu proteins are located only into mitochondria, supporting the existence of an Isu-independent Fe–S assembly machinery in plant plastids.

Mots clés: Plant; Iron–sulfur cluster; Mitochondria; Plastid

Domaines: Biologie végétale

S. Léon, B. Touraine, J.F. Briat, S. Lobréaux. Mitochondrial localization of Arabidopsis thaliana Isu Fe–S scaffold proteins. FEBS Letters, 2005, 579 (9), pp.1930-1934. ⟨10.1016/j.febslet.2005.02.038⟩. ⟨hal-00086594⟩