Static and MAS solid-state study of supported phospholipid bilayer cylindrically oriented.
Résumé
Solid-state NMR spectroscopy has been used to investigate a biomimetic model membrane. In this model, the supported bilayers, obtained by fusion of SUVs, are tethered inside nanoporous aluminum oxide supports via a streptavidin/biotin spacer arm. The static and slow Magic-Angle Spinning deuterium NMR spectra of the tethered bilayers have been compared to the experimental and simulated spectra of a simpler model with cylindrical geometry, namely a phospholipid bilayer adsorbed on an oriented polymer (PET) sheet. MAS experimental spectra of both models are in good agreement with the simulated spectrum of an ideal cylindrical distribution of phospholipids. By studying bilayers of DMPC d54, the 2H MAS experiments permit to assess the geometrical arrangement of the lipids inside the nanopores.