A new route to trihydroxamate-containing artificial siderophores and synthesis of a new fluorescent probe.

H. Ouchetto (1) , Marylène Dias (1) , René Mornet (1) , Emmanuel Lesuisse (2) , Jean-Michel Camadro (2)

1 CIMMA - Chimie, Ingénierie Moléculaire et Matériaux d'Angers
2 IJM (UMR_7592) - Institut Jacques Monod

A fluorescent labelled artificial siderophore 1 was synthesized by coupling a 7-nitrobenz-2-oxa-1,3-diazole (NBD) derivative to the terminal amino group of a new trihydroxamate-containing amine 2, a ferrichrome-type siderophore that was obtained from tris(hydroxymethyl) amino methane. Compound I was shown to be a suitable tool for experiments on siderophore transport and uptake processes in various organisms cells and particularly in Candida albicans cells.

Domaines: Biochimie, Biologie Moléculaire

H. Ouchetto, Marylène Dias, René Mornet, Emmanuel Lesuisse, Jean-Michel Camadro. A new route to trihydroxamate-containing artificial siderophores and synthesis of a new fluorescent probe.. Bioorganic and Medicinal Chemistry, 2005, 13, pp.1799-1803. ⟨10.1016/j.bmc.2004.11.053⟩. ⟨hal-00019257⟩