Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation.

Anne-Garlonn Desbrosses-Fonrouge (1) , Katrin Voigt (1) , Astrid Schröder (1) , Stéphanie Arrivault (1) , Sébastien Thomine (2) , Ute Krämer (1)

1 MPI-MP - Max Planck Institute of Molecular Plant Physiology
2 ISV - Institut des sciences du végétal

The Arabidopsis thaliana metal tolerance protein 1 (MTP1) of the cation diffusion facilitator family of membrane transport proteins can mediate the detoxification of Zn in Arabidopsis and yeast. Xenopus laevis oocytes expressing AtMTP1 accumulate more Zn than oocytes expressing the AtMTP1D94A mutant or water-injected oocytes. An AtMTP1-GFP fusion protein localizes to the vacuolar membrane in root and leaf cells. The analysis of Arabidopsis transformed with a promoter-GUS construct suggests that AtMTP1 is not produced throughout the plant, but primarily in the subpopulation of dividing, differentiating and expanding cells. RNA interference-mediated silencing of AtMTP1 causes Zn hypersensitivity and a reduction in Zn concentrations in vegetative plant tissues.

Mots clés: Cation efflux; Intron-spliced hairpin; ZAT; Zinc; Metal tolerance; Hyperaccumulation

Domaines: Autre [q-bio.OT]

Anne-Garlonn Desbrosses-Fonrouge, Katrin Voigt, Astrid Schröder, Stéphanie Arrivault, Sébastien Thomine, et al.. Arabidopsis thaliana MTP1 is a Zn transporter in the vacuolar membrane which mediates Zn detoxification and drives leaf Zn accumulation.. FEBS Letters, 2005, 579, pp.4165-4174. ⟨10.1016/j.febslet.2005.06.046⟩. ⟨hal-00018963⟩