Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages

Raíssa Volpatto Marques (1) , Stefania Enza Sestito (2) , Frédéric Bourgaud (3) , Sissi Miguel (3) , Frédéric Cailotto (2) , Pascal Reboul (2) , Jean-Yves Jouzeau (2) , Sophie Rahuel-Clermont (2) , Sandrine Boschi-Muller (2) , Henrik Toft Simonsen (1) , David Moulin (2)

1 DTU - Danmarks Tekniske Universitet = Technical University of Denmark
2 IMoPA - Ingénierie Moléculaire et Physiopathologie Articulaire
3 Plant Advanced Technologies S.A.

Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of Dicranum majus and Thuidium delicatulum inhibited NO production in a concentration-dependent manner with IC50 values of 1.04 and 1.54 µg/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.

Domaines: Sciences du Vivant [q-bio]

Raíssa Volpatto Marques, Stefania Enza Sestito, Frédéric Bourgaud, Sissi Miguel, Frédéric Cailotto, et al.. Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages. Molecules, 2022, 27 (6), pp.1940. ⟨10.3390/molecules27061940⟩. ⟨hal-03652855⟩