Study of radiation effects on the cell structure and evaluation of the dose delivered by x-ray and a-particles microscopy

E. Kosior , P. Cloetens (1) , Guillaume Devès (2) , R. Ortega (2) , S. Bohic (1, 3)

1 ESRF - European Synchrotron Radiation Facility
2 IPCV - Interface Physique et Chimie pour le Vivant
3 GIN - Grenoble Institut des Neurosciences

Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to study radiation effects on cells. Experiments were performed on freeze-dried cells at the nano-imaging station ID22NI of the European synchrotron radiation facility. Quantitative phase contrast imaging provides maps of the projected mass and is used to evaluate the structural changes due to irradiation during X-ray fluorescence experiments. Complementary to phase contrast imaging, scanning transmission ion microscopy is performed and doses of all the experiments are compared. We demonstrate the sensitivity of the proposed approach to study radiation-induced damage at the sub-cellular level.

Domaines: Biophysique [physics.bio-ph]

E. Kosior, P. Cloetens, Guillaume Devès, R. Ortega, S. Bohic. Study of radiation effects on the cell structure and evaluation of the dose delivered by x-ray and a-particles microscopy. Applied Physics Letters, 2012, 101, pp.263102. ⟨10.1063/1.4773181⟩. ⟨in2p3-00771027⟩