Physiological importance of the connective tissue in the human amnion. Role of magnesium.

M. Bara (1) , P. Moretto (2) , J. Durlach , A. Guiet-Bara (1)

1 PP - Physiologie et physiopathologie
2 CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan

The elemental ionic distribution in the epithelial layer (EL) and in connective tissue (CT = compact layer + fibroblast layer) of the human amniotic membrane has been studied in reference samples, after conservation in a physiological fluid (Hanks' solution) and after addition of 2 mM MgCl2 in Hanks' solution. Particle induced X-ray emission and Rutherford backscattering spectrometry techniques were used to provide quantitative measurements. In physiological fluid, with regard to reference samples, the monovalent ions (Na+, K +, Cl-) concentrations were identical on both layers. This data indicates that the connective tissue, in particular the compact layer, acts as a buffer which fix minerals. Mg2+ and Ca2+ levels were higher in EL than in CT. The addition of MgCl2 in Hanks' solution induced a decrease of the monovalent ion concentrations in both layers except Na+ level in EL which remained constant, an increase of the Mg2+ level in both layers, while the Ca2+ remained constant. These data indicate the possible role of connective tissue in pregnancies complicated by poly or oligohydramnios.

Domaines: Physique Médicale [physics.med-ph]

M. Bara, P. Moretto, J. Durlach, A. Guiet-Bara. Physiological importance of the connective tissue in the human amnion. Role of magnesium.. Magnesium Research, 2003, 16, pp.35-42. ⟨in2p3-00103477⟩