Reconstructed human epidermis: A model to study the barrier function

Yves Barbotteau (1) , E. Gontier (1) , P. Barberet (1) , M. Cappadoro , B. de Wever , C. Habchi (1) , S. Incerti (1) , A. Mavon , P. Moretto (1) , T. Pouthier (1) , R.W. Smith (1) , M.D. Ynsa (1)

1 CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan

The use of in vitro reconstructed human epidermis (RHE) by the cosmetic and pharmaceutical industries is increasing because of its similar physiological mechanisms to native human skin. With the advent of ethic laws on animal experimentation, RHE provides an helpful alternative for the test of formulations. The aim of this study is to check that the RHE mineral status is comparable to that of human native skin by investigating the elemental distributions in the epidermis strata. In addition, possible deleterious effects of the transport on the epidermis ionic content were studied by nuclear microscopy.

Domaines: Physique Médicale [physics.med-ph]

Yves Barbotteau, E. Gontier, P. Barberet, M. Cappadoro, B. de Wever, et al.. Reconstructed human epidermis: A model to study the barrier function. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2005, 231, pp.286-291. ⟨10.1016/j.nimb.2005.01.072⟩. ⟨in2p3-00024129⟩