Separation of Coffee Pulp Bioactive Phenolic Compounds by MPLC Fractionation and Identification by HPLC-ESI-MS
Résumé
Coffee pulp is the major by-product of the coffee industry, and its generation and accumulation is a big problem of contamination. Some strategies have been studied in order to reduce it. One of the most common strategies is turning waste into value-added products. In this study, the MPLC technique was successfully applied to the fractionation of the aqueous acetone extracts (AAE) from the coffee pulp. Seven fractions were obtained using MPLC. The fractions were characterized by reversed-phase HPLC-UV and ESI-MS. Four fractions were obtained as pure compounds according to the HPLC chromatogram. The ESI-MS showed that the main compound present in the acetone extract was caffeine. Additionally, some 218new masses observed (MW: 232, 357, and 532) had not been reported in the coffee pulp. The MS2 analysis allowed obtaining the main fragments of these new masses. Finally, these results provide new information about the characterization of some new compounds present in the coffee pulp.