REM1.3's phospho-status defines its plasma membrane nanodomain organization and activity in restricting PVX cell-to-cell movement

Artemis Perraki (1) , Julien Gronnier (1) , Paul Gouguet (1) , Marie Boudsocq (2) , Anne-Flore Deroubaix (1) , Vincent Simon (3) , Sylvie German-Retana (3) , Anthony Legrand (1, 4) , Birgit Habenstein (4) , Cyril Zipfel (5) , Emmanuelle Bayer (1) , Sébastien Mongrand (1) , Veronique Germain (3)

1 LBM - Laboratoire de biogenèse membranaire
2 IPS2 (UMR_9213 / UMR_1403) - Institut des Sciences des Plantes de Paris-Saclay
3 BFP - Biologie du fruit et pathologie
4 CBMN - Chimie et Biologie des Membranes et des Nanoobjets
5 UZH - Universität Zürich [Zürich] = University of Zurich

Plants respond to pathogens through dynamic regulation of plasma membrane-bound sig-naling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that the Arabidopsis thaliana CALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINs in vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3's phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmo-desmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses.

Domaines: Phytopathologie et phytopharmacie; Immunologie

Artemis Perraki, Julien Gronnier, Paul Gouguet, Marie Boudsocq, Anne-Flore Deroubaix, et al.. REM1.3's phospho-status defines its plasma membrane nanodomain organization and activity in restricting PVX cell-to-cell movement. PLoS Pathogens, 2018, 14 (11), pp.e1007378. ⟨10.1371/journal.ppat.1007378⟩. ⟨hal-02159426⟩