In Situ detection and single cell quantification of metal oxide nanoparticles using nuclear microprobe analysis

Giovanna Muggiolu (1) , Marina Simon (1) , Nathanael Lampe (1) , Guillaume Devès (1) , Philippe Barberet (1) , Claire Michelet (1) , Marie-Hélène Delville (2) , Hervé Seznec (1)

1 CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan
2 ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux

We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.

Domaines: Matériaux

Giovanna Muggiolu, Marina Simon, Nathanael Lampe, Guillaume Devès, Philippe Barberet, et al.. In Situ detection and single cell quantification of metal oxide nanoparticles using nuclear microprobe analysis. Journal of visualized experiments : JoVE, 2018, 132, e55041 (9 p.). ⟨10.3791/55041⟩. ⟨hal-01717535⟩