Amperometric Polyphenol Biosensor Based on Tyrosinase Immobilization on CoAl Layered Double Hydroxide Thins Films

A. Soussou (1, 2) , I. Gammoudi (2) , F. Moroté (2) , M. Mathelié-Guinlet (2) , A. Kalboussi (3) , Z.M. Baccar (1) , T. Cohen-Bouhacina (2) , C. Grauby-Heywang (2)

1 INRAP - Institut National de Recherche et d'Analyse Physico-Chimique
2 LOMA - Laboratoire Ondes et Matière d'Aquitaine
3 Laboratoire de Microélectronique et Instrumentations [Monastir]

An amperometric biosensor based on tyrosinase immobilized on the sensor surface has been used for the detection of polyphenols extracted from green tea. The immobilization was ensured by the crosslinking method on thins films of CoAlSO 4 layered double hydroxide recovering screen-printed gold electrodes. Electrochemical measurements show that this biosensor is able to detect tea polyphenols by following the reduction of compounds enzymatically generated. Its response is linear in the concentration range of [0-2,4 µM] with high sensitivity and stability, since it retains 90% of its original response after 20 days.

Mots clés: amperometric biosensor; layered double hydroxide; tyrosinase; atomic force microscopy

Domaines: Chimie analytique; Biotechnologies

A. Soussou, I. Gammoudi, F. Moroté, M. Mathelié-Guinlet, A. Kalboussi, et al.. Amperometric Polyphenol Biosensor Based on Tyrosinase Immobilization on CoAl Layered Double Hydroxide Thins Films. Procedia Engineering, 2016, 168, pp.1131-1134. ⟨10.1016/j.proeng.2016.11.371⟩. ⟨hal-01477810⟩