Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode

Yosra Braham (1) , Houcine Barhoumi (1) , Abderrazak Maaref (1) , Amina Bakhrouf (2) , Christine Grauby-Heywang (3) , Touria Cohen-Bouhacina (3) , Nicole Jaffrezic-Renault (4)

1 LIMA - Laboratoire des Interfaces et Matériaux Avancés [Monastir]
2 LATVPEP - Laboratoire d’analyse, de traitement et de valorisation des polluants de l’environnement et des produits [Monastir]
3 LOMA - Laboratoire Ondes et Matière d'Aquitaine
4 SA - Sciences Analytiques

In this work we describe a new urea biosensor, based on the immobilization of bacteria, Proteus mirabilis on gold electrode. To improve the stability of the bio-system, additional materials were used such as functionalized Fe 3 O 4 nanoparticles (NPs), cationic (PAH), anionic (PSS) polyelectrolytes, Bovine Serum Albumin (BSA) and glutaralde-hyde as a cross-linking agent. The electrochemical performances of the developed bacteria biosensor was evaluated using the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry measurements. The adhesion of the bacteria cell on gold electrode was evaluated using contact angle measurements. The morphology of bacteria and its interaction with Fe 3 O 4 nanoparticles were evaluated with the atomic force microscopy (AFM). As a result, a sensitive, stable and reproducible urea biosensor was developed.

Mots clés: Proteus mirabilis; Fe3O4; PAH and PSS polyelectrolytes; Impedance spectroscopy; Gold electrodes; Bacterial adhesion; Atomic Force Microscopy

Domaines: Biotechnologies; Chimie analytique

Yosra Braham, Houcine Barhoumi, Abderrazak Maaref, Amina Bakhrouf, Christine Grauby-Heywang, et al.. Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode. Journal of Biosensors & Bioelectronics, 2015, 6 (1), pp.1000160. ⟨10.4172/2155-6210.1000160⟩. ⟨hal-01406863⟩