Physico-chemical and antibacterial studies on silver doped nano-hydroxyapatite

S. Ciobanu C. (1) , Ecaterina Andronescu (2) , M. Prodan A. (2) , Liv Pall (2, 3) , A. Costescu (4, 5, 6) , Philippe Le Coustumer (5) , Frédéric Huneau (6) , L. Marutescu (7) , N. E. Ene (7) , R. Trusca (8) , S. Barna E. (4) , Simona Liliana Iconaru (1, 4)

1 National Institute of Materials Physics
2 Department of Science and Engineering of Oxide Materials and Nanomaterials
3 ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux
4 Faculty of Physics
5 ENSEGID - Géoressources & Environnement
6 SPE - Sciences pour l'environnement
7 Faculty of Biology
8 SC Metav-CD SA

In this paper we show that preparation of Ag doped hydroxyapatite by an adapted co-precipitation method at 100°C has several advantages over other techniques. Specifically, it can generate highly crystalline nanopowder Ag:HAp which could be used for implantable medical devices. The XRD of HAp (xAg = 0) and Ag:HAp (xAg = 0.05, and xAg = 0.4) also demonstrates that powders obtained by co-precipitation at 1000C exhibit the apatite characteristics with good crystal structure and no new phase or impurity is found. The SEM results suggested that Ag+ doping had little influence on the morphology and dimension of the samples. It can be seen that all the samples consist of elipsoidal particles. The antibactericidal activity of Ag:HAp-NPs with xAg = 0, xAg = 0.05, and xAg = 0.4 on Bacilus and E.coli ESBL 1576 were presented. The Ag:HAp-NPs with xAg = 0.05, and xAg = 0.4 inhibited the biofilm development both by the gram-positive (Staphylococcus aureus 0364) and the gram-negative (Providencia stuartii 1116) strains. On the other hand, our studies have shown that Ag:HAp with xAg = 0 had no antibacterial activity against gram-positive and gram-negative bacteria.

Mots clés: Silver; Hydroxyapatite; Antibacterial activity

Domaines: Matériaux

S. Ciobanu C., Ecaterina Andronescu, M. Prodan A., Liv Pall, A. Costescu, et al.. Physico-chemical and antibacterial studies on silver doped nano-hydroxyapatite. Journal of Optoelectronics and Advanced Materials, 2013, 15 (7-8), pp.918-922. ⟨hal-00865853⟩