Impact of RGD peptide density grafted onto polyethylene terephthalate on MC3T3 cell attachment

Céline Chollet (1) , Christine Labrugère (2) , Marie-Christine Durrieu (1)

1 Biomateriaux et Reparation Tissulaire
2 ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux

The aim of this study was to evaluate the impact of different densities on MC3T3 cells attachment onto polyethylene terephthalate (PET) film surfaces. Biomimetic modifications were performed by means of a three-step reaction procedure: creation of COOH functions onto PET surface, coupling agent grafting and finally immobilization of peptides. The originality of this work consist, in one hand on quantifying RGD peptides densities grafted onto PET, and on the other hand on studying MC3T3 cells responses after seeding on such biomimetic surfaces. After each functionnalization step, modifications were validated by several physicochemical techniques: X-Ray Photoelectron Spectroscopy permitted to prove the grafting and high-resolution β-imager coupled with use of radiolabelled amino acids served in evaluation of peptides densities. Moreover, this last technique permit us to ensure stability of binding between peptides and polymer. The efficiency of this new route for biomimetic modification of PET surface was demonstrated by measuring the adhesion at 15 hours of osteoblast like cells. Study of cellular comportment was realized by means of focal contact proteins (vinculin, actin) immunostaining.

Mots clés: Biomaterials; Grafting; Polyethylene terephthalate; RGD peptides

Domaines: Matériaux

Céline Chollet, Christine Labrugère, Marie-Christine Durrieu. Impact of RGD peptide density grafted onto polyethylene terephthalate on MC3T3 cell attachment. ITBM RBM, 2008, 29 (1), pp.7-12. ⟨10.1016/j.rbmret.2007.12.003⟩. ⟨hal-00275230⟩