RGD peptides micro-patterning on poly(ethylene terephthalate) surfaces

Céline Chollet (1) , Sylvain Lazare (2) , Christine Labrugère (3) , Fabien Guillemot (1) , Reine Bareille (1) , Marie-Christine Durrieu (1)

1 Biomateriaux et Reparation Tissulaire
2 LPCM - Laboratoire de Physico-Chimie Moléculaire
3 ICMCB - Institut de Chimie de la Matière Condensée de Bordeaux

he aim of this study was to evaluate the impact of RGD micro-patterned poly(ethylene terephthalate) (PET) on human osteoblast progenitor (HOP) cells attachment. Biomimetic modifications were performed by means of a four-step reaction: surface hydrolysis, oxidation in order to create COOH functions, coupling agent grafting (EDC, NHS) and finally immobilization of peptides. In addition to homogeneous or statistically distribution of peptides, micro-patterns of RGD were generated by: optical photolithography and UV excimer laser ablation. Modification steps were validated by physico-chemical techniques: XPS was used to prove covalent grafting at each stage of the surface functionalization, toluidine blue assay and high resolution µ-imager (using [3H]-Lys) to evaluate peptide densities and validate micro-patterns formation. Finally, the efficiency of this biomodification of PET was demonstrated onto homogeneous surfaces by measuring the adhesion between 1 and 24 h of osteoprogenitor cells isolated from HBMSC.

Mots clés: Biomaterials; Grafting; Micro-patterning; RGD

Domaines: Matériaux; Biotechnologies; Ingénierie biomédicale

Céline Chollet, Sylvain Lazare, Christine Labrugère, Fabien Guillemot, Reine Bareille, et al.. RGD peptides micro-patterning on poly(ethylene terephthalate) surfaces. ITBM RBM, 2007, 28 (1), pp.2-12. ⟨10.1016/j.rbmret.2007.03.002⟩. ⟨hal-00185252⟩