In-air scanning transmission ion microscopy of cultured cancer cells

Richard Ortega (1) , Guillaume Devès (1) , Philippe Moretto (2)

1 CNAB - Chimie Nucléaire Analytique et Bio-environnementale
2 CENBG - Centre d'Etudes Nucléaires de Bordeaux Gradignan

Scanning transmission ion microscopy (STIM) imaging of living cultured cells has been carried out using a proton external-beam with the nuclear microprobe of Bordeaux-Gradignan. STIM could be performed in air atmosphere after passage of a focused proton beam through a 150 nm thick silicon nitride window. Energy loss STIM images were obtained with a spatial resolution in the micrometer range and enabled the identification of sub-cellular ultrastructures.

Mots clés: Cell imaging; Living cells; Nuclear microprobe; STIM

Domaines: Instrumentations et Détecteurs [physics.ins-det]

Richard Ortega, Guillaume Devès, Philippe Moretto. In-air scanning transmission ion microscopy of cultured cancer cells. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2001, 181 (1-4), pp.475-479. ⟨10.1016/S0168-583X(01)00475-X⟩. ⟨hal-00139985⟩