Surface charges effects on the 2D conformation of supercoiled DNA

Tatiana Schmatko (1) , Pierre Muller (1) , Mounir Maaloum (1)

1 ICS - Institut Charles Sadron

We have adsorbed plasmid PuC19 DNA on a supported bilayer. The mobility of the lipids within the bilayer ensured a 2D equilibrium of the DNA molecule. By varying the fraction of cationic lipids in the membrane, we have tuned the surface charge. Plasmids conformations were imaged by Atomic Force Microscopy (AFM).We performed two sets of experiments: deposition from salt free solution on charged bilayers and deposition from salty solutions on neutral bilayers. Plasmids can be seen as rings, completely opened structures, or tightly supercoiled plectonemes, depending on the experimental conditions. The plectonemic conformation is observed either on charged surfaces (in the absence of salt) or at 30 mM salt concentration on a neutral bilayer. We demonstrate the equivalence of surface screening by mobile interfacial charges and bulk screening from salt ions.

Mots clés: DNA supercoiling; plasmids adsorption; membranes

Domaines: Biophysique; Biophysique [physics.bio-ph]; Matière Molle [cond-mat.soft]

Tatiana Schmatko, Pierre Muller, Mounir Maaloum. Surface charges effects on the 2D conformation of supercoiled DNA. 2012. ⟨hal-00696100v1⟩