Clinical significance and prognostic value of Chromatin Assembly Factor-1 overexpression in human solid tumors
Résumé
Aims: The chromatin assembly factor CAF-1, whose function is critical for maintaining chromatin stability during DNA replication and repair, has been identified as a proliferation marker in breast cancer. Here, we investigate the interest of CAF-1 as a proliferation marker in a wide spectrum of solid tumors, and assess its potential value in predicting clinical outcome. Methods and Results: Using immunocytochemistry on paraffin-embedded tissue sections, we compare CAF-1 labeling index with known proliferation markers Ki-67 and MCMs, and we analyze its association with clinicopathological data and patients' outcome. CAF-1 expression shows a strong positive correlation with Ki-67, routinely used to detect proliferating cells, while it generally displays weaker correlations with MCM markers, known to label cells with replicative potential. CAF-1 expression is significantly associated with histological grade in breast, cervical, endometrial and renal cell carcinomas, and with disease stage in endometrial and renal carcinomas. Furthermore, high expression of CAF-1 is an independent predictor of adverse clinical outcome in renal, endometrial and cervical carcinomas. Conclusions: These data demonstrate that CAF-1 is a proliferation marker in various malignant tumors with prognostic value in renal, endometrial and cervical carcinomas, which supports the relevance of CAF-1 as a clinical marker to monitor cancer progression.
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