Validation of a wide spectrum microbiological tube test, the EXPLORER test, for the detection of antimicrobials in muscle from different animal species
Résumé
EXPLORERâ is a simple and fast new kit for detection of inhibitory substances in raw meat. The test, a 96-well microtiter plate, is based on the inhibition of microbial growth (Geobacillus stearothermophilus spores). The Explorerâ test was validated in accordance with the decision 2002/657/EC (EC 2002). Specificity, detection capabilities for 5 compounds from major antimicrobials families and robustness were studied. Specificity of the test was tested with 4 different animal species and was very satisfactory (false positive rates lower than 10 %). The detection capabilities for amoxicillin (10 µg kg-1) and tylosin (100 µg kg-1) were at the MRL level (50 and 100 µg kg-1 respectively) for amoxicillin and tylosinboth, at 2 times the MRL for doxycycline (200 µg kg-1) and sulfathiazole (200 µg kg-1) at 2 times the MRL (100 µg kg-1 for each) and 2.5 times the MRL for cefalexin (500 µg kg-1) at 2.5 times the MRL (200 µg kg-1). 21 samples have been analysed in parallel with the Four Plate Test, the STAR protocol and the ExplorerÒ test. One false positive result and 2 false negative results (samples containing oxytetracycline) were reported with the ExplorerÒ test. In conclusion, Explorerâ test is a robust method, easily automated. Photometric reading allows informatic data storage and objective readings between technicians and days. The Explorer® test could be used as a wide screening test because it allowed to detect most of the antimicrobial families (penicillins, cephalosporins, tetracyclines, sulphonamides and macrolides) in muscles from different animal species (porcine, bovine, ovine, poultry).
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