A Sensitive Gas Chromatographic-Mass Spectrometric (C-MS) Method for the Determination of Bisphenol A in Rice-Prepared Dishes

Alberto Zafra-Gómez (1) , Juan Carlos Morales (2) , Oscar Antonio Ballesteros (1) , Alberto Navalon (1)

1 Analytical Chemistry
2 Instituto de Investigaciones Químicas, CSIC

Certain chemicals possess the potential to modulate endocrine systems, and thereby interfere with reproductive and developmental processes. Bisphenol A is suspected to be one of them. The compound is widely used as a plastic additive, lacquer, resin, or plastic and can usually be found in food samples. An accurate and reproducible gas chromatographic-mass spectrometric (GC-MS) method to detect and measure trace amounts of the compound in rice-prepared dishes samples is proposed. A solid-liquid extraction with acetonitrile was carried out in order to isolate and preconcentrate the analyte. Later, solvent was removed and a silylation step using N,O-bis(trimethylsilyl)trifluoro acetamide/pyridine (BSTFA/PYR) was carried out. The silylated compound was identified and quantified by GC-MS using a DB-5 MS column. Bisphenol F was used as a surrogate. The detection limit found was 2.0 ng/g while inter- and intra-day variability was under 6%. Due to the absence of reference materials, the method was validated using standard addition calibration and a recovery assay. Recoveries for spiked samples were over 90% and under 105%

Mots clés: Life Sciences

Alberto Zafra-Gómez, Juan Carlos Morales, Oscar Antonio Ballesteros, Alberto Navalon. A Sensitive Gas Chromatographic-Mass Spectrometric (C-MS) Method for the Determination of Bisphenol A in Rice-Prepared Dishes. Food Additives and Contaminants, 2009, 26 (08), pp.1209-1216. ⟨10.1080/02652030902939663⟩. ⟨hal-00573867⟩