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Adipose-derived stromal cells: cytokine expression and immune cell contaminants.

Abstract : The present method describes an immunoselection/depletion approach to isolate the native human adipose tissue-derived progenitor cells that are free from endothelial cells and immune cells by the use of magnetic nanobeads and microbeads coupled to antibodies. Moreover, methods to isolate and to analyse the distinct cell populations that constitute the microenvironment of the human adipose tissue progenitor cells, i.e. mature adipocytes, endothelial cells, and macrophages, are mentioned.
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Contributor : Marie Francoise Simon Connect in order to contact the contributor
Submitted on : Tuesday, August 20, 2013 - 3:23:50 PM
Last modification on : Monday, July 4, 2022 - 8:48:49 AM




Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, et al.. Adipose-derived stromal cells: cytokine expression and immune cell contaminants.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2011, 702, pp.151-61. ⟨10.1007/978-1-61737-960-4_12⟩. ⟨inserm-00852298⟩



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