Influence of oxygen tension on CD133 phenotype in human glioma cell cultures.

Nadine Platet (1) , Shi Yong Liu (1) , Michèle El Atifi (1) , Lisa Oliver (2) , François M. Vallette (2) , François R. Berger (1) , Didier Wion (1)

1 GIN - Grenoble Institut des Neurosciences
2 Recherches en cancérologie

Under standard culture conditions, tumor cells are exposed to 20% O(2), whereas the mean tumor oxygen levels within the tumor are much lower. We demonstrate, using low-passaged human tumor cell cultures established from glioma, that a reduction in the oxygen level in these cell cultures dramatically increases the percentage of CD133 expressing cells.

Mots clés: cell culture; CD133; glioma; cancer stem cell

Domaines: Neurosciences [q-bio.NC]; Cancer

Nadine Platet, Shi Yong Liu, Michèle El Atifi, Lisa Oliver, François M. Vallette, et al.. Influence of oxygen tension on CD133 phenotype in human glioma cell cultures.. Cancer Letters, 2007, 258 (2), pp.286-90. ⟨10.1016/j.canlet.2007.09.012⟩. ⟨inserm-00382779⟩