INTERALIGN: interactive alignment editor for distantly related protein sequences

Olivier Pible (1) , Gilles Imbert (1) , J-L Pellequer (1, 2)

1 SBTN - Service de Biochimie et Toxicologie Nucléaire
2 LIRM - Laboratoire des Intéractions et Reconnaissances Moléculaires

Improving and ascertaining the quality of a multiple sequence alignment is a very challenging step in protein sequence analysis. This is particularly the case when dealing with sequences in the 'twilight zone', i.e. sharing <30% identity. Here we describe INTERALIGN, a dedicated user-friendly alignment editor including a view of secondary structures and a synchronized display of carbon alpha traces of corresponding protein structures. Profile alignment, using CLUSTALW, is implemented to improve the alignment of a sequence of unknown structure with the visually optimized structural alignment as compared with a standard multiple sequence alignment. Tree-based ordering further helps in identifying the structure closest to a given sequence. Availability: Windows and Linux packages, as well as source files, are available under the CeCILL free software licensing agreement

Domaines: Sciences du Vivant [q-bio]

Olivier Pible, Gilles Imbert, J-L Pellequer. INTERALIGN: interactive alignment editor for distantly related protein sequences. Bioinformatics, 2005, 21, pp.3166 - 3167. ⟨10.1093/bioinformatics/bti474⟩. ⟨hal-03562047⟩