Chemotactic Behavior of Pathogenic and Nonpathogenic Leptospira Species

Ambroise Lambert (1) , Célia Fontana , Nadia Benaroudj , David Gasparini , Olivier Gorgette , Nathalie Cachet , Natalia Bomchil , Mathieu Picardeau , Naoko Takahashi , Nyles Charon

1 ERRMECe - Equipe de recherche sur les relations matrice extracellulaire-cellules

ABSTRACT We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B 12 were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.

Domaines: Organisation et fonctions cellulaires [q-bio.SC]; Biophysique; Biologie moléculaire; Cancer; Bactériologie; Maladies infectieuses

Ambroise Lambert, Célia Fontana, Nadia Benaroudj, David Gasparini, Olivier Gorgette, et al.. Chemotactic Behavior of Pathogenic and Nonpathogenic Leptospira Species. Applied and Environmental Microbiology, 2012, 78 (23), pp.8467-8469. ⟨10.1128/AEM.02288-12⟩. ⟨hal-03514484⟩