Intrathecal Transplantation of Autologous and Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells in Dogs

Felipe Pérez Benavides (1) , Giovana Boff Araujo Pinto (1) , Marta Cristina Thomas Heckler (1) , Diana Milena Rodríguez Hurtado (1) , Livia Ramos Teixeira (1) , Marina Mitie de Souza Monobe (1) , Gisele Fabrino Machado (1) , Guilherme Dias de Melo (1) , Diego Noé Rodríguez-Sánchez (1) , Fernanda da Cruz Landim E Alvarenga (1) , Rogério Martins Amorim (1)

1 UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University

The route used in the transplantation of mesenchymal stem cells (MSCs) can directly affect the treatment success. The transplantation of MSCs via the intrathecal (IT) route can be an important therapeutic strategy for neurological disorders. The objective of this study was to evaluate the safety and feasibility of the IT transplantation of autologous (Auto-MSCs) and allogeneic (Allo-MSCs) bone marrow mesenchymal stem cells (BM-MSCs) in healthy dogs. Based on neurodisability score, cerebrospinal fluid (CSF) and magnetic resonance imaging (MRI), no significant differences from the control group were observed on day 1 or day 5 after IT Auto- or Allo-MSCs transplantation ( P > 0.05). In addition, analysis of matrix metalloproteinase (MMP)-2 and MMP-9 expression in the CSF revealed no significant differences ( P > 0.05) at 5 days after IT transplantation in the Auto- or Allo-MSCs group when compared to the control. Intrathecal transplantation of BM-MSCs in dogs provides a safe, easy and minimally invasive route for the use of cell-based therapeutics in central nervous system diseases.

Domaines: Neurobiologie

Felipe Pérez Benavides, Giovana Boff Araujo Pinto, Marta Cristina Thomas Heckler, Diana Milena Rodríguez Hurtado, Livia Ramos Teixeira, et al.. Intrathecal Transplantation of Autologous and Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells in Dogs. Cell Transplantation, 2021, 30, pp.096368972110344. ⟨10.1177/09636897211034464⟩. ⟨hal-03504205⟩