A novel NADPH-dependent aldehyde reductase gene from Vigna radiata confers resistance to the grapevine fungal toxin eutypine.

Pedro Guillén , Monique Guis , Gracia Martinez-Reina , Ségolène Colrat , Sylvie Dalmayrac , Corine Deswarte , Mondher Bouzayen (1) , Jean-Paul Roustan (1) , Jean Fallot , Jean-Claude Pech (1) , Alain Latché (1)

1 GBF - Génomique et Biotechnologie des Fruits

Eutypine, 4-hydroxy-3-(3-methyl-3-butene-1-ynyl) benzyl aldehyde, is a toxin produced by Eutypa lata, the causal agent of eutypa dieback of grapevines. It has previously been demonstrated that tolerance of some cultivars to this disease was correlated with their capacity to convert eutypine to the corresponding alcohol, eutypinol, which lacks phytotoxicity. We have thus purified to homogeneity a protein from Vigna radiata that exhibited eutypinereducing activity and have isolated the corresponding cDNA. This encodes an NADPH-dependent reductase of 36 kDa that we have named Vigna radiata eutypine-reducing enzyme (VR-ERE), based on the capacity of a recombinant form of the protein to reduce eutypine into eutypinol. The strongest homologies (86.8%) of VR-ERE at the amino acid level were found with CPRD14, a drought-inducible gene of unknown function, isolated from Vigna unguiculata and with an aromatic alcohol dehydrogenase (71.7%) from Eucalyptus gunnii. Biochemical characterization of VR-ERE revealed that a variety of compounds containing an aldehyde group can act as substrates. However, the highest affinity was observed with 3-substituted benzaldehydes. Expression of a VR-ERE transgene in Vitis vinifera cells cultured in vitro conferred resistance to the toxin. This discovery opens up new biotechnological approaches for the generation of grapevines resistant to eutypa dieback.

Mots clés: Host agent relation; Mycotoxin; Phytotoxicity; Sensitivity resistance; Metabolism; Enzymatic activity; Oxidoreductases; Complementary DNA; Sequencing; Vigna radiata; Homology; Substrate specificity; Genetic transformation; Cell culture; Gene; Disease resistance; Toxin; Enzyme; Leguminosae; Dicotyledones; Angiospermae; Spermatophyta; Grain legume; Plant pathogen; Ascomycetes; Fungi; Thallophyta

Domaines: Agronomie; Biologie végétale; Biotechnologies

Pedro Guillén, Monique Guis, Gracia Martinez-Reina, Ségolène Colrat, Sylvie Dalmayrac, et al.. A novel NADPH-dependent aldehyde reductase gene from Vigna radiata confers resistance to the grapevine fungal toxin eutypine.. Plant Journal, 1998, 1 (3), pp.335-343. ⟨10.1046/j.1365-313x.1998.00303.x⟩. ⟨hal-03485305⟩