Genome Analysis of Haplotype D of Candidatus Liberibacter Solanacearum

Leron Katsir , Ruan Zhepu , Diego Santos Garcia (1) , Alon Piasezky , Jiandong Jiang , Noa Sela , Shiri Freilich , Ofir Bahar

1 HUJ - The Hebrew University of Jerusalem

Candidatus Liberibacter solanacearum (Lso) haplotype D (LsoD) is a suspected bacterial pathogen, spread by the phloem-feeding psyllid Bactericera trigonica Hodkinson and found to infect carrot plants throughout the Mediterranean. Haplotype D is one of six haplotypes of Lso that each have specific and overlapping host preferences, disease symptoms, and psyllid vectors. Genotyping of rRNA genes has allowed for tracking the haplotype diversity of Lso and genome sequencing of several haplotypes has been performed to advance a comprehensive understanding of Lso diseases and of the phylogenetic relationships among the haplotypes. To further pursue that aim we have sequenced the genome of LsoD from its psyllid vector and report here its draft genome. Genome-based single nucleotide polymorphism analysis indicates LsoD is most closely related to the A haplotype. Genomic features and the metabolic potential of LsoD are assessed in relation to Lso haplotypes A, B, and C, as well as the facultative strain Liberibacter crescens. We identify genes unique to haplotype D as well as putative secreted effectors that may play a role in disease characteristics specific to this haplotype of Lso.

Mots clés: genomics; fastidious bacteria; plant pathogen; Liberibacter; psyllid

Domaines: Sciences du Vivant [q-bio]

Leron Katsir, Ruan Zhepu, Diego Santos Garcia, Alon Piasezky, Jiandong Jiang, et al.. Genome Analysis of Haplotype D of Candidatus Liberibacter Solanacearum. Frontiers in Microbiology, 2018, 9, ⟨10.3389/fmicb.2018.02933⟩. ⟨hal-03270209⟩