Effect of methylene blue on the formation of oxidized phospholipid vesicles

Jean-François Fabre (1) , Muriel Cerny (1) , Audrey Cassen (1) , Zéphirin Mouloungui (1)

1 CAI - Chimie Agro-Industrielle

Soybean phosphatidylcholine, which is rich in linoleic acid, was oxidized with singlet oxygen through photosensitization with methylene blue. This compound facilitates the oxidation of phospholipids relative to the reaction with free unsaturated fatty acids. A response surface methodology was used to control oxidation, with methylene blue concentration and the amount of available air as independent variables. The conjugated diene-to triene ratio was then monitored. Hydroperoxide yield dependent principally on the amount of air, whereas photosensitizer concentration strongly influenced the size and zeta potential of vesicles formed by the sonication of oxidized phospholipids in water. Methylene blue plays an important role in the surface charge expression and ion permeability of these vesicles.

Mots clés: Methylene blue; Phospholipids; Singlet oxygen; Oxidation; Liposomes; Zeta potential; Permeability; Soybean

Domaines: Ingénierie biomédicale

Jean-François Fabre, Muriel Cerny, Audrey Cassen, Zéphirin Mouloungui. Effect of methylene blue on the formation of oxidized phospholipid vesicles. 2020. ⟨hal-03037371⟩