Gastric IL-1β, IL-8, and IL-17A expression in Moroccan patients infected with Helicobacter pylori may be a predictive signature of severe pathological stages

Ahmed Outlioua (1, 2) , Wafaa Badre , Christophe Desterke (3) , Zerif Echarki (4) , Nadira El Hammani (1) , Monsef Rabhi , Myriam Riyad , Mehdi Karkouri , Damien Arnoult (2) , Abdelouahed Khalil (3) , Khadija Akarid (1)

1 FSAC - Faculté des Sciences Aïn Chock [Casablanca]
2 Interactions cellules souches-niches : physiologie, tumeurs et réparation tissulaire
3 Service de Chirurgie générale et digestive [CHU Le Kremlin-Bicêtre]
4 UdeS - Faculté de médecine et des sciences de la santé [Sherbrooke]

Helicobacter pylori induces acute gastritis that can progress to serious diseases such as gastric cancer. H. pylori interacts with host cells within the gastric mucosa, resulting in activation of multiple innate immune signalling pathways, leading to pro- inflammatory cytokines production and immune cells recruitment. Various studies have shown that there are ethnic- and population-related differences in the expression of these cytokines. Although the H. pylori infection is a major public health problem in Morocco, to our knowledge, no study has been carried out in gastric cytokine expression from H. pylori-infected Moroccan patients. Thus we aimed to i) determine the IL- 1β, IL-8 and IL-17A gene expression in gastric biopsies from Moroccan patients infected with H. pylori, and ii) to determine the cytokine signature of each pathological stages associated with this infection. Material and methods: 71 patients with epigastralgic pain were included in this study. The H. pylori detection on gastric biopsies was performed by histopathological and PCR analysis. The IL-1β, IL-8 and IL-17A mRNA expression in the antrun and fundus biopsies was performed by RT-qPCR. Results: The histopathological and PCR analyses revealed that 87.32% of the patients were infected with H. pylori. IL-1β mRNA expression was significantly lower in the antral mucosa of H. pylori-infected patients (p = 0.0038) than in the uninfected while there was no significant difference in the expression of IL-8 and IL-17A mRNA. The expression of the three cytokines was higher in the fundic mucosa of H. pylori-infected patients than in the uninfected patients, but only IL-8 and IL-17A expression reached statistical significance (p = 0.042 and p = 0.0179 respectively). Furthermore, the multivariate predictive analysis highlighted a cytokine signature that may predict metaplasia during the infection progression that involves a specific down-regulation of IL17A and an up-regulation of IL1β in antral and fundic metaplasia respectively.

Mots clés: Helicobacter pylori; fundus; antrum; cytokine signature; Morocco

Domaines: Biologie cellulaire

Ahmed Outlioua, Wafaa Badre, Christophe Desterke, Zerif Echarki, Nadira El Hammani, et al.. Gastric IL-1β, IL-8, and IL-17A expression in Moroccan patients infected with Helicobacter pylori may be a predictive signature of severe pathological stages. Cytokines, 2020. ⟨hal-03008966⟩