Flash Chromatography System: A Practical Tool for Demonstrating the Influence of Column Characteristics on Chromatographic Resolution
Résumé
A flash purification systemusing prepacked cartridgescontaining different silica weightandparticle sizeswas employed to demonstrate the influence of column characteristicson chromatographic resolution. This chromatographic experiment was a helpful introduction to chromatographic courses where the influence of particle sizeswas addressedonly theoreticallyand it allows students to experiencethe concept in an active learning style experiment.After running a predefined set of experiments to understand concepts, students were asked to design their own experiment to achieve the best chromatographic resolution. Moreover,the difference of mass versus concentration detectors was highlighted using Ultraviolet(UV)and Evaporative Light Scattering Detectors(ELSD).Questionnaires were also used to definethestudents' initial knowledge and to assess on what they learned following this lab.
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