Automated micro-determination by transfer analyzer of circulating alpha-amino nitrogen

Micro-dosage automatisé sur analyseur à transfert de l'azote alpha-aminé circulant.

Jean-Paul Chacornac (1) , Jacques Barnouin (1) , Marie Louise Houlier (2)

1 Unité de Recherche d'Écopathologie
2 SDAR - SDAR Occitanie Montpellier

An automated method for the determination of plasma free amino groups is described. The analysis is performed on a discrete computerized analyzer after plasma deproteinization using trichloracetic acid. The method is based on the formation of a complex between terminal NH2 radicals and 2,4,6-trinitrobenzene sulfonate (TNBS). The method is accurate (coefficient of variation = 2%), linear over a range of 0.2-7 mM/l and requires small analytic volumes; it also correlates well in the bovine with results from standard liquid chromatography (r = 0.953). With this method a single operator can determine free amino group contents of 50 samples per h.

Mots clés: METHODE AUTOMATISEE

Domaines: Science des productions animales

Jean-Paul Chacornac, Jacques Barnouin, Marie Louise Houlier. Micro-dosage automatisé sur analyseur à transfert de l'azote alpha-aminé circulant.. Reproduction Nutrition Development, 1993, 33 (2), pp.99-108. ⟨10.1051/rnd:19930202⟩. ⟨hal-02711957⟩