Skip to Main content Skip to Navigation
Journal articles

Foliar CO2 fixation in bean (Phaseolus vulgaris L.) submitted to elevated ozone: distinct changes in Rubisco and PEPc activities in relation to pigment content

Abstract : Using open-top chambers, the impact of ozone (O3) on foliar carboxylases of bean (Phaseolus vulgaris L.) was investigated. From sowing, beans were exposed to non-filtered air (NF) and NF supplied with 40 (+40) and 80 (+80) nL L_1 O3. Twenty days after emergence, primary and first trifoliate leaves were sampled. Biochemical characteristics of leaves from +40 were quite similar to those from NF. Conversely, +80 induced distinct biochemical effects in primary and first trifoliate leaves. Regarding primary leaves, +80 reduced ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity by 33% whereas it stimulated phosphoenolpyruvate carboxylase (PEPc) activity by 376%. The reduction in Rubisco activity was accompanied by a decrease in both Rubisco subunit amounts and a consistent oxidative modification of the Rubisco small subunit (SSU). These changes came with a drastic loss in pigmentation. Regarding first trifoliate leaves, +80 stimulated Rubisco activity by 33% while it disturbed neither PEPc activity nor pigmentation. Surprisingly, the enhanced Rubisco activity was associated with a slight decrease in Rubisco protein quantity, which was not coupled with the formation of carbonyl groups in Rubisco-SSU.
Document type :
Journal articles
Complete list of metadatas

https://hal.inrae.fr/hal-02662987
Contributor : Migration Prodinra <>
Submitted on : Sunday, May 31, 2020 - 1:43:12 AM
Last modification on : Thursday, November 5, 2020 - 9:01:06 AM

Identifiers

Collections

Citation

Louis Leitao, Pierre Dizengremel, Jean-Philippe Biolley. Foliar CO2 fixation in bean (Phaseolus vulgaris L.) submitted to elevated ozone: distinct changes in Rubisco and PEPc activities in relation to pigment content. Ecotoxicology and Environmental Safety, Elsevier, 2008, 69 (3), pp.531-540. ⟨10.1016/j.ecoenv.2006.10.010⟩. ⟨hal-02662987⟩

Share

Metrics

Record views

14