Localization of deformed wing virus infection in queen and drone Apis mellifera L

Julie Fievet (1) , Diana Tentcheva (1) , Laurent Gauthier (1) , Joachim de Miranda (2) , Francois F. Cousserans (1) , Marc Edouard Colin (1) , Max Bergoin (1)

1 BIVI - Biologie Intégrative et Virologie des Insectes [Univ. de Montpellier II]
2 Department of Entomology

The distribution of deformed wing virus infection within the honey bee reproductive castes (queens, drones) was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3' portion of the DWV genome were used to identify DWV RNA, while a monospecific antibody to the DWV-VPI structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the whole body, including queen ovaries, queen fat body and drone seminal vesicles

Mots clés: APIS MELLIFERA L; VIRUS; RT-PCR; DWV; HISTOLOGIE

Mots clés: INSECTE; BIOLOGIE DU DEVELOPPEMENT

Domaines: Virologie

Julie Fievet, Diana Tentcheva, Laurent Gauthier, Joachim de Miranda, Francois F. Cousserans, et al.. Localization of deformed wing virus infection in queen and drone Apis mellifera L. Virology Journal, 2006, 3, pp.16. ⟨10.1186/1743-422X-3-16⟩. ⟨hal-02661809⟩