Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco)

Yann Froelicher (1) , Dominique Dambier (1) , Jean-Baptiste Bassene (1) , Gilles Costantino (2) , Samir Lotfy (3) , Christophe Didout (4) , Vincent Beaumont (4) , Philippe Brottier (5) , Ange-Marie Risterucci (1) , Luro François (2) , Patrick Ollitrault (1)

1 Cirad - Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2 Station de recherches agronomiques de San Giuliano
3 INRA Maroc - Institut national de la recherche agronomique [Maroc]
4 Centre de recherche Pernod Ricard
5 *

A dinucleotide-enriched genomic library was obtained from mandarin orange (Citrus reticulata Blanco). A subset of 101 positive clones was sequenced and primers were designed. The loci were screened for levels of variation using 26-29 wild mandarin oranges collected in Vietnam. Forty-three loci were polymorphic with the number of alleles ranging from two to 18. The observed heterozygosity (H-O) and expected heterozygosity (H-E) were from 0.03 to 0.96 and from 0.03 to 0.92, respectively.

Mots clés: CITRUS; GENETIC; DIVERSITY; MOLECULAR MARKER; RUTACEAE; STMS; MANDARINIER

Mots clés: MICROSATELLITE; CARTOGRAPHIE GENETIQUE; GENOMIQUE; GENETIQUE VEGETALE

Domaines: Ecologie, Environnement

Yann Froelicher, Dominique Dambier, Jean-Baptiste Bassene, Gilles Costantino, Samir Lotfy, et al.. Characterization of microsatellite markers in mandarin orange (Citrus reticulata Blanco). Molecular Ecology Resources, 2008, 8 (1), pp.119-122. ⟨10.1111/j.1471-8286.2007.01893.x⟩. ⟨hal-02658608⟩