Biomarkers of meat tenderness: Present knowledge and perspectives in regards to our current understanding of the mechanisms involved

Ahmed Ouali (1) , Mohammed Gagaoua (1, 2) , Yasmine Boudida (2) , Samira Becila (2) , Abdelghani Boudjellal (2) , Carlos Herrera Mendez (1) , Miguel A. Sentandreu (3)

1 QuaPA - Qualité des Produits Animaux
2 INATAA
3 CSIC - Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain]

Biomarkers of the meat quality are of prime importance for meat industry, which has to satisfy consumers' expectations and, for them, meat tenderness is and will remain the primary and most important quality attribute. The tenderization of meat starts immediately after animal death with the onset of apoptosis followed by a cooperative action of endogenous proteolytic systems. Before consideration of the biomarkers identified so far, we present here some new features on the apoptotic process. Among them, the most important is the recent discovery of a complex family of serpins capable to inhibit, in a pseudo-irreversible manner, caspases, the major enzymes responsible of cell dismantling during apoptosis. The biomarkers so far identified have been then sorted and grouped according to their common biological functions. All of them refer to a series of biological pathways including glycolytic and oxidative energy production, cell detoxification, protease inhibition and production of Heat Shock Proteins. Some unusual biomarkers are also presented: annexins, galectins and peroxiredoxins. On this basis, a detailed analysis of these metabolic pathways allowed us to identify some domains of interest for future investigations. It was thus emphasized that mitochondria, an important organelle in the production of energy from carbohydrates, lipids and proteins are a central element in the initiation and development of apoptosis. It was therefore stressed forward that, in fact, very little is known about the postmortem fate of these organelles and their multiple associated activities. Other topics discussed here would provide avenues for the future in the context of identifying reliable predictors of the ultimate meat tenderness. (C) 2013 Elsevier Ltd. All rights reserved.

Mots clés: Biomarkers; Tenderness; Metabolism; Meat quality; LONGISSIMUS-THORACIS MUSCLE; BOVINE SKELETAL-MUSCLE; HEAT-SHOCK PROTEINS; 2-DIMENSIONAL GEL-ELECTROPHORESIS; AMINO-ACID-METABOLISM; CELL-DEATH; INDUCED APOPTOSIS; MEMBRANE PERMEABILIZATION

Mots clés: Muscle cell; Apoptosis; INTRAMUSCULAR CONNECTIVE-TISSUE; MITOCHONDRIAL FISSION

Domaines: Ingénierie des aliments; Génie des procédés

Ahmed Ouali, Mohammed Gagaoua, Yasmine Boudida, Samira Becila, Abdelghani Boudjellal, et al.. Biomarkers of meat tenderness: Present knowledge and perspectives in regards to our current understanding of the mechanisms involved. Meat Science, 2013, 95 (4), pp.854 - 870. ⟨10.1016/j.meatsci.2013.05.010⟩. ⟨hal-02652573⟩