Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii

M. Million , Marie M. Maraninchi (1, 2) , M. Henry , F. Armougom , H. Richet , P. Carrieri (3, 4, 5) , Ana Valero Rello (1, 2) , D. Raccah (6) , B. Vialettes (2) , D. Raoult (4)

1 NORT - Nutrition, obésité et risque thrombotique
2 TIMONE - Hôpital de la Timone [CHU - APHM]
3 SESSTIM - U912 INSERM - Aix Marseille Univ - IRD - Sciences Economiques et Sociales de la Santé & Traitement de l'Information Médicale
4 Université de la Méditerranée - Aix-Marseille 2
5 ORS PACA
6 Hôpitaux Sud - Hôpital Sainte-Marguerite [CHU - APHM]

Background: Obesity is associated with increased health risk and has been associated with alterations in bacterial gut microbiota, with mainly a reduction in Bacteroidetes, but few data exist at the genus and species level. It has been reported that the Lactobacillus and Bifidobacterium genus representatives may have a critical role in weight regulation as an anti-obesity effect in experimental models and humans, or as a growth-promoter effect in agriculture depending on the strains. Objectives and methods: To confirm reported gut alterations and test whether Lactobacillus or Bifidobacterium species found in the human gut are associated with obesity or lean status, we analyzed the stools of 68 obese and 47 controls targeting Firmicutes, Bacteroidetes, Methanobrevibacter smithii, Lactococcus lactis, Bifidobacterium animalis and seven species of Lactobacillus by quantitative PCR (qPCR) and culture on a Lactobacillus-selective medium. Findings: In qPCR, B. animalis (odds ratio (OR) 0.63; 95% confidence interval (CI) 0.39-1.01; P = 0.056) and M. smithii (OR = 0.76; 95% CI 0.59-0.97; P = 0.03) were associated with normal weight whereas Lactobacillus reuteri (OR = 1.79; 95% CI 1.03-3.10; P = 0.04) was associated with obesity. Conclusion: The gut microbiota associated with human obesity is depleted in M. smithii. Some Bifidobacterium or Lactobacillus species were associated with normal weight (B. animalis) while others (L. reuteri) were associated with obesity. Therefore, gut microbiota composition at the species level is related to body weight and obesity, which might be of relevance for further studies and the management of obesity. These results must be considered cautiously because it is the first study to date that links specific species of Lactobacillus with obesity in humans. International Journal of Obesity (2012) 36, 817-825; doi:10.1038/ijo.2011.153; published online 9 August 2011

Mots clés: Methanobrevibacter smithii; Bifidobacterium animalis; BODY-MASS INDEX; CASEI STRAIN SHIROTA; DIET-INDUCED OBESITY; LINOLEIC-ACID; HUMAN FECES; WEIGHT; MICE; BACTERIA

Mots clés: gut microbiota; Lactobacillus reuteri; PROBIOTICS; INFLAMMATION

Domaines: Sciences du Vivant [q-bio]

M. Million, Marie M. Maraninchi, M. Henry, F. Armougom, H. Richet, et al.. Obesity-associated gut microbiota is enriched in Lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii. International Journal of Obesity, 2012, 36 (6), pp.817 - 825. ⟨10.1038/ijo.2011.153⟩. ⟨hal-02649498⟩